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M4 ; Acute myelomonocytic

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Presentation on theme: "M4 ; Acute myelomonocytic"— Presentation transcript:

1 M4 ; Acute myelomonocytic
leukemia M5 ; Acute monoblastic leukemia 1

2 - - +++ - ~ + - ~ + - ++ - ~  -  Esterase stain - - +++ - ~ + +
종류 기 질 NaF 첨가유무 호중구 단구 림프구 거핵구 Non-specific esterase Specific esterase -naphthyl acetate -naphthyl butyrate ~ ~ + - ~  ~ + - ~ + ~ + - Naphthol AS-D chloroacetate ~  

3

4 M6 ; Acute erythroblatic M7 ; Acute megakaryoblastic
leukemia M7 ; Acute megakaryoblastic leukemia 4

5 ALL (Acute lymphoblastic leukemia)

6 PAS stain Glycogen Oxidation by Periodic acid Aldehyde Schiff ’s reagent (Pararosaniline chloride 와 Sodium metabisulfite 혼합액) Reduction Purple-Red pigment

7

8 CML

9

10

11 ; leukocyte alkaline phospatase
LAP stain ; leukocyte alkaline phospatase - LAP 효소활성을 말초혈액의 호중구에서 인지 - 만성골수성 백혈병(CML)과 백혈병양반응(Leukemoid reaction)을 감별하기 위해

12

13 CLL Smudge cell, Basket cell

14 TRAP ACP (Acid Phosphatase) stain T cell ALL – red color positive
주석산(Tartaric acid)을 첨가 Hairy Cell Leukemia - Hairy cell이 주석산 저항성 양성 TRAP

15 Prussian blue 철적아구성 빈혈(sideroblastic anemia)의 진단
heme 합성, 철대사 이상 환형 철아구(ringed sideroblast)

16 Haemostasis

17 지혈에 관여하는 5가지 혈관 혈소판 혈액응고기전 섬유소용해기전 응고와 섬유소용해를 억제하는 조절인자

18 혈관

19 혈소판

20 응고인자

21 Endomitosis => Polyploidy(다배수체)
8N or 16N 8N or 16N Endomitosis => Polyploidy(다배수체)


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