1,3-Propanediol 분리/정제 공정 개발

Presentation on theme: "1,3-Propanediol 분리/정제 공정 개발"— Presentation transcript:

1 1,3-Propanediol 분리/정제 공정 개발
Department of Chemical Engineering, Kongju National University, Kongju , Korea Biochemical Engineering Lab.

2 기존 1,3-Propanediol 정제공정  Distillation - Energy intensive process
- Neither economical nor suitable (Glucose is thermally sensitive)  Zeolite Membrane → Pervaporation - Low flux and selectivity  Cation Exchange Chromatography → SMB - Purity 80%, Yield 98% - Need additional process for high purity ※ 물에 대한 용해도 높음 → 적용기술 제한 → 신규공정 개발 한계 Biochemical Engineering Lab.

3 Biochemical Engineering Lab.
분석방법 정립 1,3-Propanediol 1,2-Propanediol Glycerol Glucose  장치 / Column : HPLC (Waters) / 6.5 mm X 300 mm, CHO820 (Transgenomics)  이동상 : Distilled Water  유속 : 0.4 ml/min  주입량 : 20 µl Biochemical Engineering Lab.

4 Biochemical Engineering Lab.
크로마토그래피 정제 Purity= 100%, Yield= 73% Purity= 95%, Yield= 95% Purity= 91%, Yield= 100%  Column / Resin : 2cm x 180cm / Silica gel (0.040 ~ 0.063mm, MERCK) Elution : Ethyl Acetate / MeOH = 98 / 2 (v/v) → Isocratic elution  Flow Rate : 10 ml/min Biochemical Engineering Lab.

5 Process Scheme for Purification
원심분리 감압증발 Fermentation Broth Phase Separation Chromatography (Silica Gel) 감압증발 용매 재사용 Sugar (Biological Method) → 1,3-Propanediol (Monomer) → 3GT(Novel Polyester) 1,3-Propanediol Biochemical Engineering Lab.