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Version Space의 실험적 고찰 장해만
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Introduction DNA structure Hybirdization time version space
Other methods
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DNA structure biochemistry 4th edition p
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Hybirdization time Tm value의 측정 ds strand : 40% less than ss at 260nm
biochemistry 4th edition p990 Tm value의 측정 ds strand : 40% less than ss at 260nm Kinetic of reassociation S : single strand S`: 상보적 seq. D : ds seq. K : 평형상수 Co: 전체DNA농도 f : ss decreases
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Hybridization time
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Hybridization time Cot0.5 is directly related to the size of genome
Low con. of complementary seq slow rate of reassociation Cot0.5 is directly related to the size of genome ex) mammalian genome(30억bp) ====104 Ms 10-4m sol. of DNA ===== 108 Ms (about 3 years) 10000도시 TSP : 모든 도시를 DNA로 표현 = 47 =16384; 적어도 7bp가 소요.. 7 * (weight제외) + weight(10000!) + ,,,
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Version space Graph for All Hypothesis Edge Vertex
cs-T’ faculty-H’ Graph for All Hypothesis cs-T’ staff-H’ cs-T’ five-H’ Vertex cs staff five faculty four ee cs-T’ four-H’ cs-H cs-T ee-T’ faculty-H’ ee-H ee-T ee-T’ staff-H’ faculty-H faculty-T ee-T’ five-H’ staff-H staff-T ee-T’ four-H’ four-H four-T faculty-T’ four-H’ five-H five-T faculty-T’ five-H’ staff-T’ four-H’ staff-T’ five-H’ + Dummy strand for blunt end?
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Version Space available method 1 negative example ???
vertex 와 edge(PNA)를 이용, 가능한 모든 pool 합성 positive seq. 제작 hybridization in pool PAGE로 위치확인 gel elution PCR , 적정농도의 pool 형성 ---1st generalize 2~6 반복 ( 단, positive일 경우) negative example ??? DNA breaks down in gel elution
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Version Space available method 2 농도차이에 의한 부정확한 구분?? 비용 / 비능률
가능한 모든 pool을 주문 positive seq. 제작 (linked bead) hybridization in pool bead selection enz.처리 blunt end 형성 denaturation st generalize pool negative seq. 제작(linked bead) pool을 일부 균등분할 / hybridize / selection / enz. cutting / PAGE PAGE에서 나온 band sequencing negative probe (linked bead) 제작 1st generalize pool과 hybrid / bead selection / discard 농도차이에 의한 부정확한 구분?? 비용 / 비능률
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Other Methods Megaclone Megasort Megatype Mpss
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