Double & Triple Blocking by DNA Binding Protein

Presentation on theme: "Double & Triple Blocking by DNA Binding Protein"— Presentation transcript:

1 Double & Triple Blocking by DNA Binding Protein
비특이적 증폭 ‘Zero’에 도전 TransStart ® Hot Start Technology Antibody와 chemical 방식 보다 높은 특이성 No Primer dimer (기존 방식과의 차이점) No Mammalian DNA 오염 (Antibody 방식의 단점) No long denaturing step (Chemical 방식의 단점) DNA Binding Protein Primer Blocking Template Blocking Polymerase Blocking Double & Triple Blocking by DNA Binding Protein Primers Template Binding protein이 primer에 결합 Non-specific binding과 extension 방지 Binding protein과 template 결합 Polymerase의 template binding 방해 초기 변성 단계에서 Binding protein 파괴 Primer가 template에 결합 PCR 반응 진행 Binding Protein Polymerase Taq Polymerase TransFast® Taq EasyTaq® TransTaq® -T TransTaq® HiFi TransStart® Top Taq Efficiency & Specificity + ++ +++ ++++ +++++ Fidelity (vs EasyTaq®) 1x 18x Extension Rate 6 kb/min 1~2 kb/min 1~ 2 kb/min Hot Start △ (Genetically modified Hot start) O (Double) O (Triple) 3'-A tail O Product Size (Human gDNA) ≤ 4 kb ≤ 8 kb ≤ 15 kb GC-rich & complex template Master Mix type X Others Single Taq Taq + pfu (Enzyme Mixture) High Fidelity EasyPfu TransStart® FastPfu TransStart® FastPfu Fly Fidelity (vs EasyTaq®) 18x 54x 108x Extension Rate 0.5 kb/min 2~4 kb/min 2~6 kb/min Hot Start X O (Double) 3'-A tail Product Size (Human gDNA) ≤ 6 kb ≤ 15 kb Product Size (Plasmid DNA) ≤ 10 kb ≤ 20 kb Master Mix type O

2 DNA Polymerase Series 바이올 (Bioall) ● 일정한 결과 ● 고품질 ● 편안한 가격
DNA Binding Protein & Hot Start PCR DNA Polymerase Series ● 일정한 결과 ● 고품질 ● 편안한 가격 세계적 유명 메이커에 완제품 형태로 납품 하는 브랜드로 대부분의 제품이 유명 메이커의 엄격한 품질 기준에 부합 DNA Polymerase Product Name Code Size Price Note EasyTaq® DNA Polymerase AP111-11 500 U 50,000 General Taq (~ 4 kb) 2×EasyTaq® PCR SuperMix AS111-02 5×1 ml 157,000 TransTaq®-T DNA Polymerase AP122-11 250 U 70,000 Blended Taq (Taq + pfu) 2×TransTaq®-T PCR SuperMix AS122-02 294,000 TransTaq® DNA Polymerase HiFi AP131-11 90,000 Long & Complex (Taq-T + pfu plus) 2×TransTaq® HiFi PCR SuperMix I AS131-02 307,000 For Genomic DNA 2×TransTaq® HiFi PCR SuperMix II AS131-22 For plasmid, λDNA, cDNA TransStart® Taq DNA Polymerase AP141-11 116,000 Double Blocking Hot start TransStart® TopTaq DNA Polymerase AP151-11 141,000 Blended Taq (Taq upgrade + pfu) Triple Blocking Hot start EasyPfu DNA Polymerase AP211-11 64,000 High fidelity 2×EasyPfu PCR SuperMix AS211-02 TransStart® FastPfu DNA Polymerase AP221-11 173,000 Fast High fidelity (2 ~ 4 kb/min) 2×TransStart® FastPfu PCR SuperMix AS221-02 545,000 TransStart® FastPfu Fly DNA Polymerase AP231-11 231,000 Fast Higher fidelity (2 ~ 6 kb/min) SYBR Green qPCR SuperMix Product Name Code Size Price Note TransStart® Top Green qPCR SuperMix AQ131-02 5×1 ml 200,000 Double Blocking (Template / Primer) TransStart® TopTaq DNA Polymerase TransStart® Tip Green qPCR SuperMix AQ141-02 230,000 Triple Blocking 부산광역시 해운대구 반여로 83 Tel Fax 부산, 경남지역 전문 대리점 바이올 (Bioall)