제 6장 체세포배 유도 접합자배와 체세포배의 차이 체세포배 유도의 기술 이해 - 활엽수종 - 침엽수종 3. 실지 기술적용 예
Zygotic Embryogenesis
Zygotic embryogenesis : Double fertilization (Angiosperm)
Single fertilization (Gymnosperm)
접합자 배 (Zygotic embryo) 체세포 배 (Somatic embryo) : 자연수정으로 형성된 배 : 자연배와 유사한 구조 및 기능을 가진 인공종자 배
Somatic embryogenesis Difference between adventive bud and adventive embryo Adventive bud Adventive embryo 1. Monopolar shoot Bipolar organization 2. Organogenesis Embryogenesis 3. Single, multi-cellular origin Single cell origin 4. Arise from any part in vivo Within ovular tissue in vivo 5. Vascular connection with mother tissue No vascular connection 6. Shoot, root from different cell Cotyledon, radicle from same cell
The order explants from responsive to recalcitrant in the induction of somatic embryogenesis 1. Immature embryo 7. Floral organs 2. Hypocotyl (dicot.) 8. Meristems 3. Cotyledon (dicot.) 9. Endosperm 4. Scutellum (monocot.) 10. Young tissue of vegetative organs 5. Germinating mature embryo 11. Differentiated tissues of 6. Nucellus
체세포배 유도의 시초 초본류: 당근 (steward 등, 1958) 활엽수: Citrus 류 (?, 1970) 침엽수: 독일가문비 (Hakman, 1985)
활엽수종 산림생명공학 및 실습-4주차 EC formation SE induction & development Maturation Conversion Plantles
오갈피 류 체세포배 유도
침엽수종
Initiation & Proliferation Germination Maturation Initiation & Proliferation Osmoticum Nitrogen source PGR Maturation