대구경북첨단의료산업진흥재단 (DGMIF) 설치류 미세수술 모델 김희정, DVM, Ph.D 수의지원팀장, 선임연구원 hkim@dgmif.re.kr 실험동물센터 (LAC) 대구경북첨단의료산업진흥재단 (DGMIF)
The Three Rs The Three Rs (3Rs) are guiding principles for more ethical use of animals in testing. These were first described by W.M.S. Russell and R.L. Burch in 1959. The 3Rs are: Replacement which refers to the preferred use of non-animal methods over animal methods whenever it is possible to achieve the same scientific aims. These methods include computer modeling. Reduction which refers to methods that enable researchers to obtain comparable levels of information from fewer animals, or to obtain more information from the same number of animals. Refinement which refers to methods that alleviate or minimize potential pain, suffering or distress, and enhance animal welfare for the animals used. These methods include non-invasive techniques. https://www.wikipedia.org/
동물실험 시 고려점 준비단계 수술 및 실험 실험 완료 1. 임상적 질병과 유발 질환동물과의 특성 분석을 통한 동물 선택 2. 시술자의 기술 숙련도 3. 시설 1. 마취 2. 수술 3. 술후 처치 4. 동물관리 1. 동물평가 안락사 2. 분자생물학적 평가 3
질환동물모델 An animal model is a living, non-human animal used during the research and investigation of human disease, for the purpose of better understanding the disease process without the added risk of harming an actual human. -Wikipedia
설치류 수술 번식관련 수술 피하삽입수술 골손상모델제작 암세포 이식관련 수술 혈관계 수술 뇌위치 고정기사용 관련 수술
뇌졸중 동물모델 A stroke, sometimes referred to as a cerebrovascular accident (CVA), is the rapid loss of brain function due to disturbance in the blood supply to the brain. Strokes can be classified into two major categories: ischemic and hemorrhagic. Modeling Stroke (1) models not requiring craniotomy (embolic model, intraluminal suture MCAO model, photothrombosis model, and endothelin-1-induced stroke model) (2) models requiring craniotomy (Tamura’s model) (3) posterior cerebral circulation stroke models (4) cerebral venous thrombosis models
(1) MCAO stroke model Common Carotid Artery (CCA) Internal Carotid Artery (ICA) External Carotid Artery (ECA) Middle Cerebral Artery (MCA) Pterygopalatine artery (PPA) Occipital artery (OA) Middle cerebral artery occlusion (MCAO) Willis’ Circle