Development of Separation and Purification

Presentation on theme: "Development of Separation and Purification"— Presentation transcript:

1 Development of Separation and Purification
Process for Homoharringtonine from Cephalotaxus koreana Thank you for chairperson. My name is jong kwan park. This study was carried out to synthesize of Fe-Si Alloy Nanoparticles by Gas Phase Reaction. Department of Chemical Engineering, Kongju National University, Kongju , Korea

2 ABOUT Homoharringtonine
Alkaloid isolated from genus Cephalotaxus Antileukemia agent Structure of Homoharringtonine HHT contents in Cephalotaxus koreana

3 Extraction of Biomass Effect of ethanol extraction times on HHT yield
Effect of extraction time on HHT yield

4 Liquid/Liquid extraction
Effect of pH on yield in liquid/liquid extraction Phase separation in liquid/liquid extraction

5 Separation & purification process for Homoharringtonine
discard Biomass Chloroform Layer Aqueous Layer Rotary Evaporation Solvent Extraction Solid Residue Filtration Adsorption (Active clay treatment) Filtrate Filtercake Chromatography (Silica) Rotary Evaporation Aqueous Residue HPLC Liquid/Liquid Extraction with Chloroform, Four times Homoharringtonine (>98%)

6 생물공학제품 및 의약품의 분리 및 정제 공정 개발
생물자원으로부터 목적물질이 확인되면 이를 대량 생산할 수 있는 경제적이고 친환경적인 분리 및 정제 공정을 개발 (분리 및 정제 효율뿐만 아니라 용매회수 등 추후 공정에 미치는 영향도 고려)하고, 특히 최종 정제를 위한 전처리 공정 개발에 주력하고 있으며 주요 적용기술로 추출, 흡착, 침전, membrane 분리, 크로마토그래피, 건조 등이 있다. 적용 기술 분리 및 정제 단계별 크로마토그램