cyclic AMP, GMP

세포 신호전달체계 - 세포와 세포간, 세포 내에서 신호 교환. 외부자극(신호)에 대한 반응에 따라 세포의 대사, 분비, 신경전달, 성장, 분화, 세포분열, 노화, 죽음 등의 생명현상을 유지할 수 있는 시스템. 분자 수준의 질병의 작용기전을 밝힘으로써 암, 뇌 관련 질병, 면역계 질병, 혈관장애 등 질병치료제의 개발에 응용. 외부 환경의 변화나 자극에 의한 반응. 세포자극물질(agonists)은 세포를 통과하지 못해 세포막에 존재하는 수용체와 결합한 후 세포내부에 있는 신호전달체계를 활성화시키기 위한 이차신호전달물질(second messengers)을 생성 - 이차신호전달물질: cyclic AMP, cyclic GMP

cAMP (cyclic- Adenosine MonoPhosphate) 외부 자극에 의한 세포 내 신호전달 기능 (second messenger) 세포막에 위치한 adenylyl cyclase에 의해 ATP로 부터 합성 당, 지질 대사 등의 조절 기능 효소 활성화 촉진.

cGMP (cyclic- Guanidine MonoPhosphate) 외부 자극에 의한 세포 내 신호전달 기능 (second messenger) 세포막에 위치한 guanylate cyclase에 의해 GTP로 부터 합성 세포 내 protein kinase에 의해 활성 세포 내 세포사멸에 작용 혈관 확장 기능.

culture supernatants, plasma, serum, saliva, urine cGMP ELISA kit 제품종류 비교 – Enzo Products Cat.No 용도 Sample type Direct cGMP ADI-900-014 세포 내 cGMP 정량  cell lysates, tissue  cGMP ADI-900-013 세포 외 cGMP 정량 culture supernatants, plasma, serum, saliva, urine Complete cGMP ADI-900-164 세포내 & 외 cGMP 정량 cell lysates, culture supernatants, plasma, serum, saliva, tissue, urine

cAMP ELISA kit 제품종류 비교 – Enzo Products Cat.No 용도 Sample type Direct cAMP ADI-900-066 세포내 cAMP 정량 cell lysates, tissue cAMP ADI-900-067 세포외 cAMP 정량  culture supernatants, serum, saliva Complete cAMP ADI-900-166 세포내&외 cAMP 정량 cell lysates, culture supernatants, serum, saliva, tissue

#ADI-900-066 Product Literature References Constitutive cholesterol-dependent endocytosis of melanocortin-4 receptor (MC4R) Is essential to maintain receptor responsiveness to α-melanocyte-stimulating hormone (α-MSH): F.K. McDaniel, et al.; J. Biol. Chem. 287, 21873 (2012), Application(s): ELISA using human neuronal cells, Abstract; Regulation of pancreatic cancer by neuropsychological stress responses: a novel target for intervention: H.M. Schuller, et al.; Carcinogenesis 33, 191 (2012), Application(s): ELISA using human pancreatic cancer xenografts, Abstract; Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress: S.J. White, et al.; J. Cell. Physiol. 226, 2841 (2011),Application(s): ELISA using HUVEC, Abstract; Full Text cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase: Chen J., et al.; Reproduction 138, 759 (2009), Abstract; Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells: P. Hardwidge, et al. ; J. Bacteriol. 191, 178 (2009), Application(s): EIA using porcine cell lysates, Abstract; Androgens transduce the G-alpha s-mediated activation of protein kinase A in prostate cells: Y. Daaka, et al. ; Cancer Res. 68, 3225 (2008), Application(s): EIA using human cell lysates,Abstract; beta-Arrestin-1 mediates glucagon-like peptide-1 signaling to insulin secretion in cultured pancreatic beta cells: J. Olefsky, et al. ; PNAS 105, 6614 (2008), Application(s): EIA using rat cell lysates, Abstract; Cyclic Adenosine Monophosphate Regulation of Ion Transport in PorcineVocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008), (ref. 2), Abstract; FMRP acts as a key messenger for dopamine modulation in the forebrain: M. Zhao, et al. ; Neuron 59, 634 (2008), Application(s): EIA using mouse cell lysates, Abstract; Functional evidence for presence of lipid rafts in erythrocyte membranes: Gs-alpha in rafts is essential for signal transduction: Y. Takakuwa, et al. ; Am. J. Hematol. 83, 371 (2008),Application(s): EIA using human cell lysates, Abstract; Regulation of G proteins by human 5-HT1a receptor TM3/i2 and TM5/i3 loop peptides: H.V. Thiagaraj, et al. ; Neurochem. Int. 50, 109 (2007), Application(s): EIA using human cell lysates, Abstract; Co-occurrence of Two Partially Inactivating Polymorphisms of MC3R Is Associated With Pediatric-Onset Obesity: N. Feng, et al. ; Diabetes 54, 2663 (2005), Application(s): EIA using human cell lysates, Abstract; The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator: T. Petnicki-Ocwieja, et al. ; J. Bacteriol. 187, 649 (2005), Application(s): EIA using plant cell lysates, Abstract; High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al. ; Am. J. Physiol. Heart Circ. Physiol. 284, H1124 (2003), Application(s): EIA using rat cell lysates, Abstract; Lysine 270 in the Third Intracellular Domain of the Oxytocin Receptor is an Important Determinant for G{alpha}q Coupling Specificity: M. Yang, et al. ; Mol. Endocrinol. 16, 814 (2002), Application(s): EIA using monkey cell lysates, Abstract; Effect of Tumor Necrosis Factor-{{alpha}}, Interferon-{{gamma}}, and Transforming Growth Factor-{beta} on Adipogenesis and Expression of Thyrotropin Receptor in Human Orbital Preadipocyte Fibroblasts: R. W. Valyasevi, et al.; J. Clin. Endocrinol. Metab. 86, 903 (2001), (ref. 1), Abstract;