Fragment-Based Drug Discovery of 2-Thiazolidinoes as BRD4 Inhibitors : Structure – Based Optimization 2014/04/03 Jun Min Jung
Abstract
Introduction
Chemistry R1NH2 = Cyclopentanamine
Chemistry R1NH2 = Cyclopentanamine
Chemistry
Chemistry
Chemistry
Chemistry
Chemistry
Chemistry
Results & Discussion Initial Optimization
Initial Optimization
Structure – Activity relationships
Structure – Activity relationships
Structure – Activity relationships
Effects on c-myc protein and mrna expression
Western Blot Southern blotting Northern blotting Western blotting(Immunoblotting)은 antigenic epitope과 반응하는 antibody를 이용하여 항원- 항체 반응을 일으킴으로써 단백질 혼합물 중에서 원하는 특정단백질(antigen)만을 찾아내는 방법 이다 Southern blotting DNA molecules을 agarose gel로부터 membrane으로 옮긴 후, 특정적인 DNA sequence를 probe로 사용하여 detection해내는 방법 Northern blotting Northern blot은 Southern blot과 같은 원리이며, DNA 대신 특정한 RNA detection하는 방법이다.
In vitro Metabolic stability assessment
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