조혈과 혈구생성기관
Hematopoesis & hemopoietic growth factors http://blog.naver.com/hyouncho2/60045031898
Prenatal hematopoiesis
Growth factors for treatment of disease and complementing transplantation procedures: rhuEpo (recombinant human erythropoietin) End stage renal failure Anaemia of chronic disease (infection, chronic inflammation, malignancy) Expensive, does not seem to be immunogenic G-CSF/GM-CSF Bone marrow transplantation Aplastic anaemia patients Stimulates stem cells quickly M-CSF For tumour-type disorders
Cell cycle: What causes cells to continue dividing?
Neutrophil Kinetics
골수검사 적응증 ① 혈액질환이 의심되고 임파절, 간비종대 ② 말초혈액소견이 골수이형성증후군 또는 백혈병 ③ 원인을 알 수 없는 빈혈, 혈소판 감소, 백혈구 감소 ④ 방사선 소견에서 혈액질환 의심 ⑤ 암의 전이여부(metastasis) 확인 ⑥ 육아종(granuloma)의 세균학적 또는 병리조직학적 검사 ⑦ Storage disease나 골질환의 진단 ⑧ 빈혈, 백혈병 등의 치료효과와 예후 판정 ⑨ 다발성골수종의 진단 및 면역글로불린의 이상 ⑩ 철결핍성빈혈, 거구성빈혈 등 혈액질환의 추적검사, 골수배양, 세포유전학적검사 http://blog.naver.com/hyouncho2/60104608331
Aspiration/Biopsy Needle Jamshidi BM Aspiration/Biopsy Needle
Bone Marrow Sites Iliac bone Tibia(newborn)
Illinois Needle BM asirate Jamshidi Needle BM aspiration/biopsy
A : Osteocyte B : Hemopoietic cells A : Bone marrow B : Bone matrix C : Skeletal muscle
Normal Peripheral Blood Smear (정상 말초혈액 도말) Normocytic Normochromic RBCs & Platelet
Polychromatic RBCs in newborn
Supravital stain by brilliant cresyl blue Reticulocyte (망상적혈구) = Young RBC, 0.5-1.5% Polychromasia = polychromatophilia (다염색성)
CFU-GEMM CFU-GM CFU-G Myeloblast Metamyelocyte Myelocyte Promyelocyte