PIVKAII 검사의 유용성 면역혈청계 곽은민
1.Introdution Prothrombin induced by Vitamin K Absence or Antagonist-II(PIVKA-II) = DCP(Des-r-Carboxy Prothrombin) Absence of Vitamin K or Antagonist of Vitamin K Prothrombin precursor vitk dependent r-glutamic acid ----------- r – Carboxylgutamic acid r-glutamyl-carboxylase
II.PIVKA II 측정원리 Anti-Human DCP mouse monoclonal Ab가 부착되어 있는 Plate에 검체를 반응시킨후 POD 표식 Anti-human Prothrombin Rabbit IgG Ab를 반응시킨다. 검체중 DCP가 존재하면 Sandwitch complex가 형성되고 발색액을 가하여 효소반응을 시켜서 PIVKA II 농도를 구한다.
III.Materials Sera were collected from 110 patients between July 2000 and August 2000 in Shinchon Severance Hospital Group : Total 110 patients{ MIF : 89/21 Age : 56(38-81)} Sample : Serum (Plasma) Sample Volume : 100ul
IV.Methods PIVKA II : 異常 PIVKA II (E) FRS (SRL, Tokyo, Japan) AFP-L3 : LBA method (Liquid phase Binding Assay) LIBASYS Auto-analyzer (Wako, Tokyo, Japan) AFP : Electrochemiluminiscence assay ELECSYS ( Boehringer- Mannheim, Germany)
V.PIVKA II Procedure 항 PIVKA-II Monoclonal 항체고상 Plate에 측정용평형액 100ul 분주 제1반응 실온 2시간 세정 (4회) 효소표식항체 200ul 분주 제2반응 실온 1시간 세정 (4회) 발색액 200ul 분주 제3반응 실온 30분 반응정지액 50ul 분주 흡광도측정(450/650nm) 농도계산
VI.결과산출 및 보고방법 Detection Limit : 10 ~ 2000mAu/ml
PIVKAII , AFP-L3 , AFP in relation of Diaginosis
Diagnostic value of PIVKAII in HCC, in comparison with AFP-L3
Correlation between AFP,PIVKAII,AFP L3 in HCC (mAu/ml) (IU/ml)
Number of requisition for PIVKAII 6 7 8 9
VIII.Summary No significant correlation was found between PIVKA II or AFP L3 and AFP. PIVKA II and AFP L3 combined with AFP contributed to increased detection of HCC.